Protein Therapeutic Bioanalysis by Hybrid IA-LC-MS/MS: Optimization of a Preclinical Antibody Workflow, Upcoming Webinar Hosted by Xtalks

By: via PRWeb

In this free webinar, the featured speaker will give an overview of bioanalysis of protein therapeutics using a surrogate peptide approach. He will discuss case study results for the preclinical bioanalysis of a humanized IgG protein therapeutic by hybrid IA-LC-MS/MS. Attendees will learn about practical tips for optimizing immunoaffinity enrichment and trypsin digestion workflows.

TORONTO (PRWEB) June 09, 2020

Bioanalysis of protein therapeutics by LC-MS/MS continues to gain popularity as a complementary approach to ligand-binding assays (LBA). LC-MS/MS offers multiple advantages over LBA including higher specificity, multiplexing capability and reduced development time. Immunoaffinity enrichment can be coupled to LC-MS/MS to produce a hybrid method that increases sensitivity and selectivity.

This webinar will highlight the systematic optimization of a flexible, hybrid IA-LC-MS/MS workflow that uses streptavidin-coupled magnetic beads, ’rapid’ trypsin digestion and surrogate peptide quantification.

Key areas of focus will include:

  • The selection of streptavidin-coupled magnetic beads as the platform for the immunoaffinity enrichment system due to their amenability to use in combination with analyte-specific biotinylated capture reagents
  • A workflow case study for a humanized antibody therapeutic in preclinical serum using generic conserved surrogate peptides for quantification that, when optimized, ultimately requires only ~3 hours with minimal direct handling
  • Additional applications for the optimized workflow, such as highly selective and sensitive analysis of humanized antibody in preclinical samples and protein therapeutics using analyte-specific biotinylated capture reagents

Join Brendan Powers, PhD, Staff Scientist, Covance in a live webinar on Thursday, June 25, 2020 at 11am EDT (4pm BST/UK).

For more information or to register for this event, visit Protein Therapeutic Bioanalysis by Hybrid IA-LC-MS/MS: Optimization of a Preclinical Antibody Workflow.

Xtalks, powered by Honeycomb Worldwide Inc., is a leading provider of educational webinars to the global life science, food and medical device community. Every year thousands of industry practitioners (from life science, food and medical device companies, private & academic research institutions, healthcare centers, etc.) turn to Xtalks for access to quality content. Xtalks helps Life Science professionals stay current with industry developments, trends and regulations. Xtalks webinars also provide perspectives on key issues from top industry thought leaders and service providers.

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